Extended Data Fig. 2: Analysis and workflow for cryo-EM data collected on a Talos Arctica and on a Titan Krios.
From: Structural mechanism for NEK7-licensed activation of NLRP3 inflammasome
a, A negative-staining electron microscopy image (top, one among a few hundred images) and a cryo-EM micrograph (bottom, one among a few thousand images) of the NLRP3–NEK7 complex from a Talos Arctica. Scale bars, 50 nm. b, Workflow of cryo-EM data analysis of the Arctica data, performed in RELION 3.053 and cisTEM46. c, Gold-standard FSC curve between two half maps from the Arctica data. d, Local resolution estimation of the Arctica map generated by ResMap41, coloured on the cryo-EM density (8σ). The highest resolution is observed where NEK7 interacts with NLRP3. e, A cryo-EM micrograph of the NLRP3–NEK7 complex from a Titan Krios (one among more than 10,000 images). Scale bar, 50 nm. The inset shows the modelled (left) and actual (right) Thon rings. f, Workflow of cryo-EM data analysis of the Krios data, done in RELION 3.053 and ROME 1.148. The top right insets show the orientation distributions of the particles from the dataset of tilt 0° and tilt 20°. g, Gold-standard FSC curves between two half maps from the Krios data with mask (orange) and without mask (green), and between map and model (blue).
