Extended Data Fig. 9: Plasmids used in this study to construct host strain SKA703.
From: A universal biomolecular integral feedback controller for robust perfect adaptation
a, Suicide plasmids used to delete the ARA, lactose and rhamnose metabolic pathways from MG1655. The disrupted gene region and flanking upstream and downstream genomic sequences were inserted into suicide vector pRE112. The remaining deletion scar is indicated in red. b, λ-Integration plasmid used for the chromosomal integration of constitutively expressed lacYA177C. c, Tn7-integration plasmid used for the chromosomal integration of constitutively expressed tetR.
