Extended Data Fig. 5: MfLon protease-mediated degradation of AraC and sfGFP is closely matched.
From: A universal biomolecular integral feedback controller for robust perfect adaptation
Protein levels of V5-tagged AraC with a Pdt#3c degradation tag with and without aTc-induction of Mflon were compared to fluorescence levels of sfGFP with a Pdt#1 tag. a, Mean sfGFP fluorescence of the cultures used for lysate preparation. sfGFP fluorescence normalized to the predisturbance level. Error bars denote standard deviation (n = 3). Circles denote the individual replicates. b, Means of V5-tagged AraC immunoblot band intensities normalized to total protein. Band intensity normalized to the pre-disturbance level. Error bars denote standard deviation (n = 3). Circles denote the individual replicates. c, Immunoblot. Left, total protein on membrane post-transfer. Right, V5-AraC-Pdt#3c immunoblot. V5-AraC-Pdt#3c is indicated with an arrow. SKA703 was included as a no-plasmid negative control (neg ctrl). For immunoblot source data, see Supplementary Fig. 1. Data are representative of three independent experiments.
