Extended Data Fig. 7: FACS gating strategies.
From: Single-cell analysis reveals T cell infiltration in old neurogenic niches
a, FACS scheme for the isolation of PROM1+EGFR+CD45−CD31−O4−CD24a− aNSCs/NPCs from the adult SVZ. The gate shown on each plot is indicated above the plot. Marker and fluorophore are shown on each axis. b, FACS scheme for the isolation of CD8+CD4−CD3+CD45+TCRγ/δ−B220−TER119−CD11b− T cells from adult SVZ, spleen and blood. The gate shown on each plot is indicated above the plot. The marker and the fluorophore are shown on each axis.
