Extended Data Table 4 Chemoselective synthesis of N-acetyl dipeptidyl amides

From: Peptide ligation by chemoselective aminonitrile coupling in water

  1. Yields of the oxidative coupling of Ac-Gly-SH (50 mM) and AA-NH2 (150 mM) with K3[Fe(CN)6] (150 mM) in water at room temperature and pH 9.5. 1H NMR yields were determined with an internal NMR standard.
  2. aThe observed ratio of mono- and di-acylated products varies with solution pH (see Extended Data Fig. 1b for α-selectivity of Lys-NH2 ligation at pH 7.5 and Supplementary Table 12).
  3. bReaction carried out at pH 6.5 (see Supplementary Table 9).
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