Extended Data Fig. 7: RecBCD initiation kinetics at 300 μM ATP, solution pH 8.
From: Rotation tracking of genome-processing enzymes using DNA origami rotors
a, Example trajectories of initiation for the blunt-end DNA substrate and DNA substrates with 6-nt 3′ or 10-nt 5′ overhangs. Representative of at least 3 independent biological replicates. The green and magenta colour-coding is as described in Fig. 3a. b, Mean duration of initiation phase (total time from substrate binding until processive unwinding) for the blunt-end substrates and substrates with 6-nt 3′ or 10-nt 5′ overhangs. The green and magenta portions indicate the mean cumulative dwell times in the green and magenta states, respectively. Error bars are s.e.m. (n = 80, 44 and 85 (from left to right) trajectories from at least 3 independent biological replicates). Individual data points of the durations of initiation phase are overlaid as dot plots. Data were acquired at 300 μM ATP, solution pH 8, 10% glycerol, 500 Hz.
