Extended Data Fig. 2: GFP pulldown assay finds mutations for LOCKR.

Different putative LOCKR constructs were adhered via the 6×His tag to a Ni-coated 96-well plate, key–GFP was applied and excess was washed away. The resulting mean fluorescence values represent key–GFP bound to LOCKR constructs. The truncation was used as a positive control, because the key binds to the open interface. The monomer was used as a negative control, because it does not bind the key. Error bars represent the s.d. of three technical replicates (key–GFP was not purified from bacterial lysate. which led to minor technical variability).