Extended Data Fig. 2: Copy-number analysis distinguishes malignant from non-malignant single cells.
From: Resolving medulloblastoma cellular architecture by single-cell genomics
a–e, Heat maps show scRNA-seq-derived copy-number profiles of every cell in each sample (y axis) along the genome (x axis) for WNT (a), SHH (b), Group 3 (c) and Group 4 (d) patient MBs as well as PDX samples (e). Copy-number profiles derived from array-based DNA methylation profiling from the same sample are shown above. CNVs are observed in 21/25 patient tumour samples (all except MUV34, MUV41, SJ577 and SJ625). Generally, we observe a high concordance between single-cell and DNA methylation array-derived copy-number profiles. Genetic subclones at the level of broad copy-number changes are detected in samples SJ99 and BCH825. Cells without detected CNVs from samples that showed CNVs in the majority of cells are indicated for samples in which at least four non-malignant cells were detected (BCH807 and SJ454). Amplifications of the MYC and MYCN oncogenes detected by DNA methylation array are indicated.
