Extended Data Fig. 6: Inhibition of cell growth mediated by methionine restriction is largely due to interruptions to the production of nucleosides and redox balance.
From: Dietary methionine influences therapy in mouse cancer models and alters human metabolism
a, The synergic effects of methionine restriction and 5-FU treatment in CRC119 primary cells and HCT116 cells were evaluated by cell counting. Mean ± s.e.m. n = 3 biological replicates. *P < 0.05 by two-tailed Student’s t-test. b, The rescue effect of choline, formate, homocysteine, homocysteine with vitamin B12, nucleosides and NAC, alone or in combination, on the inhibition of HCT116 cell proliferation mediated by methionine restriction. Mean ± s.e.m., n = 9 biologically independent samples from 3 independent experiments. *P < 0.05 versus control, ^P < 0.05 versus methionine restriction, #P < 0.05 versus 5-FU treatment, $P < 0.05 versus methionine restriction + 5-FU treatment, by two-tailed Student’s t-test. c, Mass intensity for [M + 1] dTTP and [M + 1] methionine in HCT116 cells from the experiment described in Fig. 2h. Mean ± s.d. n = 3 biologically independent samples. *P < 0.05 versus control and #P < 0.05 versus methionine restriction, by two-tailed Student’s t-test.
