Extended Data Fig. 7: Dietary restriction of methionine sensitizes mouse models of RAS-driven autochthonous sarcoma to radiation.
From: Dietary methionine influences therapy in mouse cancer models and alters human metabolism
a, Volcano plots of metabolites in tumour, plasma and liver, and pathway analysis of metabolites significantly changed (*P < 0.05, two-tailed Student’s t-test) by dietary restriction of methionine alone (false discovery rate < 1). b, Spearman’s rank correlation coefficients of fold change of metabolites in tumour, plasma and liver induced by methionine restriction. c, Volcano plots of metabolites in tumour, plasma and liver, and pathway analysis of metabolites significantly changed (*P < 0.05, two-tailed Student’s t-test) by dietary restriction of methionine and radiation (false discovery rate < 0.5). d, Spearman’s rank correlation coefficients of fold change of metabolites in tumour, plasma and liver induced by methionine restriction and radiation. e, Relative intensity of metabolites related to cysteine and methionine metabolism, and energy balance in tumours. Mean ± s.d. n = 7 mice per group, except for the methionine-restriction group (n = 6). *P < 0.05 versus control, by two-tailed Student’s t-test. f, g, The largest effects on metabolism occurred in the combination of diet and radiation. f, Effect of methionine restriction and radiation alone, or in combination, on metabolites in tumour, plasma and liver, evaluated by taking the log10 of fold change. Box limits are the 25th and 75th percentiles, centre line is the median, and the whiskers are the minimal and maximal values. The data represent metabolites in liver (319), plasma (308) and tumour (332) from n = 7 mice per group, except for the methionine-restriction group (n = 6). g, Numbers of metabolites significantly changed (*P < 0.05, two-tailed Student’s t-test) by methionine restriction and radiation alone, or in combination.
