Extended Data Table 1 Methionine concentrations in plasma, tumour and liver across mouse models and humans

From: Dietary methionine influences therapy in mouse cancer models and alters human metabolism

  1. Tissues were collected at the end of each experiment. Concentrations in tissues were estimated in μM, assuming that 1 g wet tissue weight = 1 ml. Quantification was performed by using 13C-labelled standards for each amino acid, which were added before extraction. Cyclic loess normalization and linear regression were applied in quantification of methionine in samples without 13C-labelled standards. Values are mean ± s.d. n = 8 for CRC119, CRC240, CRC119 (vehicle) and CRC119 (5-FU), 7 for sarcoma on the control diet, 6 for sarcoma on the methionine-restricted diet, 7 for sarcoma (radiation), 5 for C57BL/6J mice and 6 for humans. *P < 0.05, by two tailed Student’s t-test between the control diet and the methionine-restricted diet; #P < 0.05, by two tailed Student’s t-test between the control diet and the methionine-restricted diet + radiation.
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