Extended Data Fig. 5: Lysine and methionine supplementation have no growth-relevant effect on the oxidative capacity of media containing H₂O₂.

a, H₂O₂ levels measured by Amplex Red fluorescence in water (left) or SM medium (right) supplemented with l-lysine (25 μg ml⁻¹), d-lysine (25 μg ml⁻¹) or l-methionine (40 μg ml⁻¹) with or without 30 min pre-incubation with 2.5 mM H₂O₂. The data represent mean ± s.d.; n = 3 independent experiments. b, Growth curves of cells pre-cultured in SM, SM + lysine (SM + Lys, 25 μg ml⁻¹) or SM + methionine (SM + Met, 40 μg ml⁻¹). At mid-log phase (OD_600nm ≈ 0.5) cells were pre-incubated for 0 or 30 min with 2.5 mM of H₂O₂ in the respective pre-culture medium. The data represent mean ± s.d.; n = 3 biologically independent samples.

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