Extended Data Fig. 1: Expression of innate immune checkpoints in human cancer.
From: CD24 signalling through macrophage Siglec-10 is a target for cancer immunotherapy
a, Heat map of expression (log2(normalized counts + 1)) of CD24 from bulk TCGA and TARGET studies, as compared to known innate immune checkpoint molecules CD47, PD-L1 and B2M (tumour study abbreviations and n values are defined in Supplementary Table 1). b, Expression levels of CD24 in ovarian cancer (OV, red box plot, n = 419) in comparison with ovarian tissue from healthy individuals (grey box plot, n = 89), boxes show the median and whiskers indicate the 95th and 5th percentiles, ****P < 0.0001, unpaired, two-tailed Student’s t-test. c, Expression levels of CD24 in TNBC (red box plot, n = 124) in comparison with ER+PR+ breast cancer (purple box plot, n = 508) and healthy breast cells (grey box plot, n = 293). Each symbol represents an individual patient sample, boxes show the median and whiskers indicate the 95th and 5th percentiles, ****P < 0.0001, one-way ANOVA with multiple comparisons correction, F(2,922) = 95.80. d, Heat map of marker gene expression (y axis) across TNBC single cells (x axis) and cell clusters identified (top). e, UMAP dimension 1 and 2 plots displaying all TNBC cells analysed from six patients (n = 1,001 single cells); cell clusters are coloured by cell patient (for key, see right). f, CD24 compared with PD-L1 expression in the ‘Tumour epithelial cell’ cluster for individual TNBC patients. Number of single cells analysed: PT039, n = 151 cells; PT058, n = 11 cells; PT081, n = 196 cells; PT084, n = 84 cells; PT089, n = 117 cells; PT126, n = 60 cells. **P < 0.01, ****P < 0.0001. Data are violin plots showing median expression (log2(normalized counts +1) and quartiles (paired, two-tailed t-test).
