Extended Data Fig. 1: Pressure chamber schematic and PIEZO1-knockout validation.
From: Mechanosensation of cyclical force by PIEZO1 is essential for innate immunity
a, Schematic of CHP bioreactor showing side view. b, Graph of pressure regimes used within the pressure chamber. c, RT–qPCR analysis of Piezo1 in unstimulated BMDMs from Piezo1fl/fl (WT) and Piezo1ΔLysM mice. Data are presented as five biological replicates from two independent experiments. d, RT–qPCR analysis of Piezo1fl/fl BMDMs treated with static pressure at the indicated magnitude or with CHP for 6 h. Data are presented as four biological replicates from two independent experiments. e, Immunoblot analysis of HIF1α and β-tubulin from Piezo1fl/fl BMDMs treated with static pressure at the indicated magnitude or with CHP for 6 h. Data are representative of two independent experiments. f, ELISA of IL-1β from Piezo1fl/fl and Piezo1ΔLysM BMDMs treated with CHP alone (NT), 10 ng ml−1 LPS with 5 h CHP followed by 1 h CHP, 5 h CHP followed by 1 h nigericin (10 μM) in CHP, or 10 ng ml−1 LPS for 5 h followed by 1 h nigericin (10 μM) with CHP. Data are presented as three biological replicates. Data in c, d, f are mean ± s.e.m.; significance is determined by unpaired two-tailed t-test (*P < 0.05, **P < 0.01, ***P < 0.001 and ****P < 0.0001).
