Extended Data Fig. 1: E-cad expression is retained during several intermediate stages of metastasis.
From: E-cadherin is required for metastasis in multiple models of breast cancer
a, E-cad expression is determined by immunofluorescence at various stages of metastasis following orthotopic transplantation of MMTV-PyMT; mT/mG tumour organoids into NSG host mice. Observations described in this figure were made across at least three independent MMTV-PyMT tumours. b, Tumour organoids embedded in collagen I express membrane-localized E-cad. Scale bar, 50 μm. c, Representative tile scan showing E-cad expression within a primary tumour section. Scale bar, 500 μm. Three selected regions within the tumour (yellow boxes; scale bar, 200 μm) express high levels of membrane-localized E-cad. d–g, E-cad is also expressed in invasion strands (d), locally disseminated units (e), intravasated units (f) and distant metastases (g) in vivo. Scale bar, 50 μm. Arrowhead, disseminated unit (e) or intravasated unit (f). Yellow box in f marks a magnified inset (scale bar, 20 μm).
