Extended Data Fig. 2: Binding affinities between conformation-specific antibodies and the native or glutaraldehyde-treated TCR–CD3 complex.
From: Structural basis of assembly of the human T cell receptor–CD3 complex
a–e, Binding affinities of native or glutaraldehyde-treated TCR–CD3 protein complex with activation antibodies OKT3 and UCHT1 and three antibodies targeting CD3γ, CD3δ and CD3ζ, measured by bio-layer interferometry. Green or blue curves are the experimental trace obtained from bio-layer interferometry experiments, and red curves are the best global fits to the data used to calculate the equilibrium-dissociation constant (Kd) values. Data are representative of three independent experiments. f, Negative staining of TCR–CD3 particles before (top) and after (bottom) cross-linking.
