Extended Data Fig. 3: FAP is expression in mouse cardiac fibroblasts after injury.
a, Immunohistochemistry co-staining of cardiac troponin (red) and FAP (green; left) and vimentin (green; right) in adjacent sections from the left ventricle of a mouse treated with AngII/PE for 2 weeks. FAP and vimentin are expressed in the same fibroblasts (arrowheads). Representative images from two independent experiments, showing similar results (n = 7 biologically independent mice). b, Immunohistochemistry of FAP (green) in various organs or tissues after 1 week of AngII/PE treatment. Representative image of n = 3 biologically independent mice, showing similar results. c, Masson’s trichrome stain for fibrosis (blue; top, centre) and immunohistochemistry of FAP (green; bottom) in coronal heart sections of a wild-type mouse 2 weeks after continuous AngII/PE treatment. Staining and immunohistochemistry were performed on adjacent sections. Bottom insets, higher magnification of left ventricular free wall. Representative image of two independent experiments, showing similar results (n = 7 biologically independent mice). d, Immunohistochemistry of FAP (green) in mouse models of cardiac injury. DMD, Duchenne’s muscular dystrophy (mdx/mTRKO G2 mice); MI, myocardial infarction; TAC, transverse aortic constriction. Scale bars, 100 μm.
