Extended Data Fig. 6: Locomotor activity of wild-type flies and flies with various genetic defects.
From: Daytime colour preference in Drosophila depends on the circadian clock and TRP channels
a, Average activity of flies in tubes with colour filters (black line) and without filters (orange line) under 12 h:12 h light-dark. Light source was set at 2,000 lx for tubes without filters. This was comparable to the irradiance of light transmitted through the filters in the colour preference experiments (Methods). The morning (M) and evening (E) bursts of activity are similar in the two experiments. However, the level of activity during the middle of the day is substantially lower in tubes with filters (n = 18 flies for each experiment). b–i, Average activities of tested genotypes with abnormal colour preference (n shows number of flies). All flies except gl60j (c) and circadian-clock mutants (d) show well-defined morning and evening bursts. The activity peaks seen in per0 and tim0 data (d) are not anticipatory morning and evening peaks, but instead are startle responses to lights turning on or off. Average activity was calculated as the distance moved by individual flies between consecutive video frames (captured at 1 frame per s) and binned over 3-min intervals. Black and white bars indicate dark and light portions of light–dark cycle.
