Extended Data Fig. 3: Characterization of reversibility of p53-dependent effects in KPsh cells.
From: α-Ketoglutarate links p53 to cell fate during tumour suppression
a, αKG/succinate ratio in KrasG12D;TRE-shRenilla (KRensh) PDAC cells cultured with or without doxycycline for the indicated number of days. b–d, Western blot for p53 (b), population doublings (c) and αKG/succinate ratio (d) in two KPsh lines cultured with or without doxycycline for six days, or cultured without doxycycline for six days followed by six days of culture with doxycycline. The arrow indicates when doxycycline was re-introduced. Experiments in a, c were performed twice with similar results, and experiments in b, d were performed once. For gel source data of), see Supplementary Fig. 1. Data are presented as mean ± s.d. of n = 3 independently treated wells of a representative experiment with individual data points shown (a, c, d), or are representative of 1 independently treated well (b).
