Extended Data Fig. 9: Lipid profiling showing a decrease in the quantity of certain classes of lipids in Irf6−/− compared to wild-type skin.
From: The RIPK4–IRF6 signalling axis safeguards epidermal differentiation and barrier function
a, Graphs of relative mRNA expression of Grhl3, Ocln, Pnpla1, Sptlc3, Cers3 and Rora in E15.5 skin. Each circle represents a different embryo, n = 3. Centre represents the mean; error bars denote s.d. Unpaired, two-tailed Fisher’s exact test with 95% confidence interval. b, Quantification of 12 classes of lipids in wild-type and Irf6−/− E16.5 skin (n = 4 each) (nmol per g of wet skin). Data are mean ± s.d. FDRs (or adjusted P value) of <0.05 are shown on the graph. P values were obtained using a moderated t-test (two-sided) with the Benjamini–Hochberg method for multiple comparisons. c, Quantification of four different species of HCERs in wild-type and Irf6−/− E16.5 skin (n = 4 each) (nmol per g of wet skin). Each dot represents one skin sample. Centre represents the mean; error bars denote s.d. FDRs of <0.05 are labelled on the graph. P values were obtained using a moderated t-test (two-sided) with the Benjamini–Hochberg method for multiple comparisons. There is a significant decrease in species with ultra-long-chain acyl moieties (≥C26) in Irf6−/− compared to wild-type skin. d, Quantification of four different species of DCERs in wild-type and Irf6−/− E16.5 skin (n = 4 each) (nmol per g of wet skin). Each dot represents one skin sample. Centre represents the mean; error bars denote s.d. FDRs of <0.05 are labelled on the graph. P values were obtained using a moderated t-test (two-sided) with the Benjamini–Hochberg method for multiple comparisons. There is a significant decrease in species with ultra-long-chain acyl moieties (≥C26) in Irf6−/− compared to wild-type skin.
