Extended Data Fig. 10: Lipid profiling showing a decrease in the quantity of certain classes of lipids in Ripk4D161N/D161N compared to wild-type skin.
From: The RIPK4–IRF6 signalling axis safeguards epidermal differentiation and barrier function
a, E18.5 skin sections from wild-type and Ripk4D161N/D161N embryos (n = 3) were stained with Nile red fluorescent dye, which indicates polar lipids in red and non-polar lipids in green. 72.66 × 72.66 microns. b, Profiling of 12 classes of lipids in wild-type and Ripk4D161N/D161N E16.5 skin. The x axis denotes the sum(log2(abundance in Ripk4D161N/D161N/abundance in wild type)) per analyte; values less than 0 indicate a decrease, and values greater than 0 an increase, in Ripk4D161N/D161N versus wild-type skin. Green bars denote a significant FDR of <0.05. Moderated t-test (two-sided). P values were obtained using a moderated t-test (two-sided) with the Benjamini–Hochberg method for multiple comparisons. There are significantly lower levels of CERs, DCERs and LCERs in Ripk4D161N/D161N compared to wild-type skin. Levels of the other classes of lipids are unchanged. c, Quantification of 12 classes of lipids in wild-type and Ripk4D161N/D161N E16.5 skin (n = 4) (nmol per g of wet skin). Each dot represents one skin sample. Centre represents the mean; error bars denote s.d. FDRs of <0.05 are shown on the graph. P values were obtained using a moderated t-test (two-sided) with the Benjamini–Hochberg method for multiple comparisons.
