Extended Data Fig. 2: Keratinocyte-specific deletion of Irf6 or Ripk4 demonstrates their cell-autonomous requirement for epidermal differentiation.
From: The RIPK4–IRF6 signalling axis safeguards epidermal differentiation and barrier function
a, Western blots of epidermis and fetal liver from an E18.5 Irf6EKO embryo (K14-Cre+; Irf6loxp/loxp) and its K14-Cre+; Irf6+/loxp littermate control. As expected, there is efficient deletion of IRF6 in the epidermis only. Representative of three independent experiments. b, Table showing the observed and expected numbers at clipping (P4–P7) of offspring that were generated from intercrossing Irf6loxp/loxp; K14-Cre− females and Irf6+/loxp; K14-Cre+ males. No Irf6EKO mice were observed. c, P0 Irf6EKO and its littermate control (n = 3 each). Scale bar, 1 cm. d, Toluidine blue staining of P0 Irf6EKO and control embryos (n = 3 each). Scale bar, 1 cm. e, E18.5 skin sections from control and Irf6EKO embryos (n = 3 each) stained with H&E or antibodies against K10, K14 and Ki67. Scale bars, 50 μm. f, E18.5 sections from control and Irf6EKO embryos (n = 3 each) stained with H&E, showing no fusion of the squamous epithelium at the mouth. Scale bars, 200 μm. g, Western blots of epidermis and whole skin from an E18.5 Ripk4EKO embryo (K14-Cre+; Ripk4loxp/loxp) and its K14-Cre+; Ripk4+/loxp littermate control. As expected, there is efficient deletion of Ripk4 in the epidermis only. Representative of three independent experiments. h, Table showing the observed and expected numbers at clipping (P4–P7) of offspring that were generated from intercrossing Ripk4loxp/loxp; K14-Cre− females and Ripk4+/loxp; K14-Cre+ males. No Ripk4EKO mice were observed. i, P0 Ripk4EKO and its littermate control (n = 3 each). Scale bar, 1 cm. j, Toluidine blue staining of P0 Ripk4EKO and control embryos (n = 3 each). Scale bar, 1 cm. k, E18.5 skin sections from control and Ripk4EKO embryos, stained with H&E or immunolabelled with antibodies against K10, K14 and Ki67. Scale bars, 50 μm. l, E18.5 sections from control and Ripk4EKO embryos stained with H&E, showing no fusion of the squamous epithelium at the mouth and no fusion of the tongue to the palate. Scale bars, 800 μm.
