Extended Data Fig. 3: The epidermis of E18.5 Ripk4D161N/D161NIrf6−/− embryos is indistinguishable from that of Irf6−/− embryos.
From: The RIPK4–IRF6 signalling axis safeguards epidermal differentiation and barrier function
a, E18.5 sections with the indicated genotypes (n = 3 each) stained with H&E, showing fusion of the oral mucosa (arrows), irregular incisors with premature eruption (asterisks) and fusion of the stratified squamous portion of the stomach in the Irf6−/− and Ripk4D161N/D161NIrf6−/− embryos. Scale bars, 200 μm (oral mucosa); 100 μm (stomach). b, E18.5 skin sections with the indicated genotypes (n = 3) immunolabelled with antibodies against K10, K14 and Ki67. Scale bars, 50 μm. c, Table showing the observed and expected numbers at clipping (P4–P7) of offspring that were generated from intercrossing Irf6+/−Ripk4+/D161N and wild-type mice. Irf6+/−Ripk4+/D161N offspring were weaned at the expected numbers.
