Extended Data Fig. 6: Mechanosensitive ion channels are required for appressorium formation but dispensable for septin-mediated cytoskeletal reorganization.
From: A sensor kinase controls turgor-driven plant infection by the rice blast fungus
a, Conidia were collected from Guy11 and inoculated on glass coverslips in the presence and absence of gadolinium (Gd+3). Appressoria were imaged by bright-field microscopy at 24 h.p.i. Addition of gadolinium disrupted appressorium formation. Images are representative of n = 3 independent biological replications of the experiment. b, Micrographs showing the cellular localization of Sep5–GFP at the appressorium pore of Guy11 after treatment with gadolinium or verapamil at 0–20 h.p.i., imaged at 24 h.p.i. Images are representative of n = 3 independent biological replicates. Scale bars, 10 μm (a, b). c, Percentage of appressoria that have intact septin rings after treatment with gadolinium and verapamil. Data are mean ± s.d. for n = 3 independent biological replicates; 100 appressoria were counted per replicate. **P < 0.01 (two-tailed unpaired Student’s t-test).
