Extended Data Fig. 1: Application of glycerol to intact rice leaves does not cause cell collapse.
From: A sensor kinase controls turgor-driven plant infection by the rice blast fungus
a, Micrographs showing epidermal strips of a transgenic rice line that expresses the plasma-membrane marker Lti6B–GFP, treated with either water or glycerol (5 M) for 24 h. Treatment with glycerol caused cell collapse. b, Intact leaves from 2-week-old Lti6B–GFP transgenic rice plants were inoculated with 30-μl drops of water or 5 M glycerol and incubated for 3 days. No plasmolysis was observed; that is, glycerol was unable to cause the collapse of cells in whole plants. c, Rice plants were treated with water or 5 M glycerol spray and incubated for 5 days (n = 3 independent replications of the experiment). Glycerol did not have any effect on the health of the rice plant or cause any wilting—confirming that no plasmolysis of rice cells from intact leaves occurs (as shown in b). Micrographs are representative of two independent replicates of the experiment. Scale bars, 20 μm (a); 5 μm (b).
