Extended Data Fig. 9: Specificity residues of novel PhoQ*–PhoP* pathways are distinct from all extant two-component signalling interfaces.
From: Engineering orthogonal signalling pathways reveals the sparse occupancy of sequence space
a, Hamming distance was calculated between the 11 specificity residues of each PhoQ*–PhoP* variant pair, and the 11 specificity residues of wild-type PhoQ–PhoP, the two-component signalling paralogues in E. coli or all extant two-component signalling proteins. When comparing two sets, all distances between all members of both sets are plotted. b, Force-directed graph representing the sequence space of histidine kinases. Each node represents a single histidine kinase, with the relative positions reflecting the similarity of their interface residues (Methods). Coloured nodes highlight specific sets of kinases, as indicated in the legend.
