Extended Data Fig. 10: Global insulation of the AQ4*–PhoP4* chimeric signalling pathway.
From: Engineering orthogonal signalling pathways reveals the sparse occupancy of sequence space
a, Phosphotransfer profile of PhoQ4*. PhoQ4* was autophosphorylated and then incubated for 5 and 60 min with each of 27 response regulators from E. coli and with PhoP4*, as in Extended Data Fig. 4c. This experiment was repeated independently twice with similar results. b, c, RNA-seq analysis, as in Extended Data Fig. 7b, c, of strains that express AQ4* and either wild-type PhoP or PhoP4* (measured after 30 min induction with 0 mM or 1 mM trans-zeatin). b, The PhoP-regulated genes yfp, mgtL and mgrB are induced by trans-zeatin only when AQ4* is paired with PhoP4*. c, The expression change of all response regulators and histidine kinases (fold change in response to 10 mM Mg2+ or 1 mM trans-zeatin).
