Extended Data Fig. 5: Insulation of E. coli histidine kinases from PhoP* variants.
From: Engineering orthogonal signalling pathways reveals the sparse occupancy of sequence space
a–e, Phosphotransfer profiles of five histidine kinases endogenous to E. coli: EnvZ (a), RstB (b), CreC (c), CpxA (d) and PhoR (e). In each case, the kinase was autophosphorylated, then incubated for 5 or 60 min. with its cognate response regulator, with wild-type PhoP, or with one of eleven PhoP* variants, and analysed as in Extended Data Fig. 4. n = 1 independent experiment.
