Extended Data Fig. 7: Properties of striatal neurons and movement thresholds.
From: Population imaging of neural activity in awake behaving mice
a, Average firing rate, size, and interspike interval (ISI) for 14 neurons recorded in 9 FOV in 2 mice. Cells simultaneously recorded in the same FOV are colour-coded (blue, red and green). Cells in rows with a white background were recorded individually. b, Selected trace from cell 9 exhibiting spike bursting (top), and a zoomed-in view of the boxed region (bottom). A.U., arbitrary unit. Identified spikes are indicated by the marks on top of the trace. c, Single frame images for FOVs with multiple neurons, colour-coded as in a. Scale bars, 20 μm. d, Representative optical traces from two Cre-dependent SomArchon-expressing striatal cholinergic interneurons in a ChAT-Cre mouse (left; scale bar, 20 μm), recorded in 3 sessions, while mouse was awake with head fixed and navigating a spherical treadmill (n = 2 neurons from 1 mouse). Top trace corresponds to top neuron on left; two bottom traces correspond to bottom neuron. Image acquisition rate, 826 Hz. e, Histogram of instantaneous movement speeds for all FOVs shown in Fig. 3 (nine FOVs in two mice). Instantaneous movement speed was calculated as average speed during each 0.5-s time interval. Red line, threshold for low movement speed identification; green line, threshold for high movement speed identification. f, Histogram of instantaneous movement speed for individual FOVs analysed.
