Extended Data Fig. 8: In vivo SomArchon performance over time in the striatum and hippocampus of awake mice.
From: Population imaging of neural activity in awake behaving mice
a–h, Average fluorescence intensity, SNR per spike, and firing rates of neurons in the striatum and hippocampus of awake mice, over multiple trials. a–c, In each striatal recording session, we performed 5 trials, each 12-s long, with inter-trial intervals of 30–60 s. Average fluorescence intensity (a) decreased slightly; spike SNR (b) and firing rates (c) remained constant throughout the recording session (repeated-measures analysis of variance (ANOVA), n = 6 neurons in 5 FOVs from 1 mouse). d–h, In each hippocampal recording session, we performed 10 trials, each 6-s long, with inter-trial intervals of 20–30 s. Average fluorescence intensity (d) showed a slight but significant decrease across trials. SNR (e) decreased between the first and second trials but not afterwards, and firing rate (f) remained constant. Spike amplitude (g) fluctuated randomly over trials, and there was a significant increase in baseline noise (h) between the first and second trials (repeated-measures ANOVA; *P < 0.05, post-hoc test: Tukey’s HSD test, n = 16 neurons in 7 FOVs from 4 mice, Supplementary Table 2). Measurements were normalized to the first trial for each neuron. Box plots as in Fig. 4. i–m, A representative continuous optical trace of a hippocampal neuron over 80 s in an awake, head-fixed mouse (i), with zoomed-in views (j–m) at the beginning and end of the recording highlighting comparable firing rates and SNRs (n = 16 neurons in 7 FOVs from 4 mice).
