Extended Data Fig. 10: Pairwise coherence and correlation measures over spatial distance.
From: Population imaging of neural activity in awake behaving mice
To investigate the potential of background fluorescence signals under wide-field imaging to produce shared crosstalk signals on neuron pairs, we examined the relationship of various coherence and correlation measures between neurons and background fluorescence over spatial distance. a, b, Pairwise coherence at theta frequencies between neurons. Vmo–Vmo coherence did not decrease significantly with spatial distance. (a, n = 25 pairs analysed with spatial distance of 11–66 μm, centre to centre; b, n = 23 pairs within 50 μm of each other; F = 1.44, P = 0.26, one-way ANOVA). c, d, Pairwise Vmo–Vmo coherence at gamma frequencies (30–50 Hz) was not dependent on spatial distance (c, n = 25 pairs; d, n = 23 pairs within 50 μm of each other; F = 2.10, P = 0.13, one-way ANOVA). e, f, Pairwise correlation between neurons did not decrease significantly with spatial distance (e, n = 25 pairs; f, n = 23 pairs within 50 μm of each other; F = 1.00, P = 0.42, one-way ANOVA). g–l, Same analysis as in a–f performed in background doughnut ROIs surrounding each neuron (Methods). Similar to results from neuron pairs, we found that theta frequency coherence between background doughnut ROIs was not dependent on spatial distance (g, n = 23 pairs; h, n = 21 pairs; F = 0.65, P = 0.59, one-way ANOVA), nor was gamma frequency coherence (i, n = 23 pairs; j, n = 21 pairs; F = 1.93, P = 0.16, one-way ANOVA), or the correlation coefficient (k, n = 23 pairs; l, n = 21 pairs; F = 1.02, P = 0.41, one-way ANOVA). m–o, The coherence between neurons and their corresponding doughnuts was not correlated at theta frequency (m), at gamma frequency (n), or for the Pearson correlation coefficients (o). Box plots are as in Fig. 4.
