Extended Data Fig. 5: Live-SIM imaging of 53BP1-MDs; workflow and dynamics in control cells.
From: Stabilization of chromatin topology safeguards genome integrity
a, Schematic depiction of live-cell 3D-SIM workflow. b, Live-SIM of a chromosome locus harbouring DNA breakage under wild-type conditions. U2OS–GFP-53BP1 cells were treated with 10 ng ml–1 NCS to induce DSBs and imaged immediately for up to 22.5 min at 2.5-min intervals. Image galleries for seven fields from four independent acquisitions are displayed. Manual classification of transition stages is colour-coded. Scale bars, 200 nm. For detailed image information see Supplementary Table 1.
