Extended Data Fig. 3: Increased evolution of CRISPR-based resistance across a range of microbial community compositions over time.

Proportion of P. aeruginosa that acquired surface modification or CRISPR-based immunity (or remained sensitive) at up to 3 d.p.i. with phage DMS3vir when grown either in monoculture (100%) or in polyculture mixtures consisting of the mixed microbial community but with varying starting percentages of P. aeruginosa based on volume (6 replicates for most samples, with 24 colonies per replicate, n = 42 biologically independent replicates for a, n = 32 biologically independent replicates for b, and n = 42 biologically independent replicates for c). a, Resistance evolution at 1 d.p.i. Data are mean ± s.e.m. Deviance test: relationship between CRISPR and P. aeruginosa starting percentage at time point 1; residual deviance (35, n = 42) = 4.42, P = 0.004; 1%; z = −3.27, P = 0.002; 10%; z = 1.21, P = 0.23; 25%; z = 1.62, P = 0.11; 50%; z = 2.20, P = 0.034; 90%; z = 2.07, P = 0.046; 99%; z = 0.47, P = 0.65; 100%; z = 1.47, P = 0.15. b, Resistance evolution at 2 d.p.i. Data are mean ± s.e.m. Deviance test: relationship between CRISPR and P. aeruginosa starting percentage at time point 2; residual deviance (25, n = 32) = 3.86, P = 2.51 × 10⁻⁶; 1%; z = −2.14, P = 0.04; 10%; z = 1.19, P = 0.25; 25%; z = 2.07, P = 0.049; 50%; z = 1.89, P = 0.07; 90%; z = 1.12, P = 0.27; 99%; z = 1.21, P = 0.24; 100%; z = 1.11, P = 0.28. c, Resistance evolution at 3 d.p.i. Data are mean ± s.e.m. Deviance test: relationship between CRISPR and P. aeruginosa starting percentage at time point 3; residual deviance (35, n = 42) = 8.24, P = 0.0004; 1%; z = −3.38, P = 0.002; 10%; z = 2.12, P = 0.04; 25%; z = 2.77, P = 0.009; 50%; z = 3.07, P = 0.004; 90%; z = 2.46, P = 0.019; 99%; z = 1.55, P = 0.13; 100%; z = 0.87, P = 0.39

Source Data.