Extended Data Figure 9: The pore interior of Tom40 and precursor import paths.
From: Structure of the mitochondrial import gate reveals distinct preprotein paths
a, b, The views from the cytosol (top) and cut-away views (bottom) for Tom40. Section planes for the cut-away views are indicated with broken lines in the top panels. The side chains of the Tom40 residues crosslinked to the translocation intermediates of a presequence-containing precursor pSu9–DHFR (a) and of a hydrophobic presequence-lacking precursor AAC–DHFR (b) are shown on the EM structure with colour reflecting the amount of crosslinked products detected4. The acidic patches (red) and hydrophobic patches (green) are shown in the cut-away views for the β-barrel side of the pore interior of Tom40. Possible precursor translocation paths are indicated with arrows by broken lines (bottom). c, Section curved plane for the cut-away views (centre, bottom) is indicated with a broken line in the view from the cytosolic side (top). The acidic patches (red), hydrophobic patches (green) and possible precursor translocation paths (broken lines) are shown (bottom).
