Extended Data Fig. 2: Effect of HSD on neurons, astrocytes, microglia/macrophages, pericytes and white-matter integrity.
From: Dietary salt promotes cognitive impairment through tau phosphorylation
a, HSD (NaCl 8%) does not affect neurons (NEUN), astrocytes (GFAP), microglia/macrophages (IBA-1) (cortex: microglia, ND/HSD n = 5/5, P = 0.0570; hippocampus: ND/HSD n = 5/5, P = 0.0556; two-tailed unpaired t-test for HSD versus ND) or pericytes (CD13) in both the pyriform cortex and the hippocampus (scale bars, 500 μm (except where specified)). b, No evidence of neuronal cell death is observed in HSD-fed mice by Fluoro-Jade B or TUNEL staining (scale bar, 500 μm). +DNase indicates positive control for TUNEL staining. Representative images from ND- and HSD-fed mice (n = 5 per group). c, Klüver–Barrera stain shows no white-matter damage in the corpus callosum of HSD-fed mice (scale bar, 100 μm). Representative images from ND- and HSD-fed mice (n = 4 per group). Data are expressed as mean ± s.e.m.
