Extended Data Fig. 9: PE3-mediated correction of E6V-encoding HBB mutation and HEXA1278+TATC by various pegRNAs.
From: Search-and-replace genome editing without double-strand breaks or donor DNA
a, Screen of 14 pegRNAs for correction of the HBB E6V-encoding allele in HEK293T cells with PE3. All pegRNAs evaluated convert the mutant HBB allele back to wild-type HBB without the introduction of any silent PAM mutation. b, Screen of 41 pegRNAs for correction of the HEXA1278+TATC allele in HEK293T cells with PE3 or PE3b. Those pegRNAs labelled HEXAs correct the pathogenic allele by a shifted 4-bp deletion that disrupts the PAM and leaves a silent mutation. Those pegRNAs labelled HEXA correct the pathogenic allele back to wild-type. Entries ending in b use an edit-specific nicking sgRNA in combination with the pegRNA (the PE3b system). Mean ± s.d. of n = 3 independent biological replicates.
