Extended Data Fig. 4: CASP8(C362S) induces necroptosis-independent tissue destruction.
From: Caspase-8 is the molecular switch for apoptosis, necroptosis and pyroptosis
a, Representative images of Casp8WT/WTMlkl−/− (n = 8), Casp8C362S/WTMlkl−/− (n = 7) and Casp8C362S/C362SMlkl−/− (n = 3) mouse embryos at E13.5 (top). Whole-mount yolk sacs stained with anti-CD31 antibody as endothelial marker (bottom). Scale bars, 100 µm. b, Representative images of spleen, inguinal and mesenteric lymph nodes from Casp8C362S/flTie2creMlkl−/− (n = 2) and Casp8fl/flMlkl−/− (n = 2) mice. c, Representative images of 9-day-old mice (top left) and skin sections stained with H&E (top right, bottom). Scale bars, 100 µm (magnification, top) and 300 µm (bottom). d, Expected, observed and weaned numbers of mice per genotype obtained from the indicated crossings.
