Extended Data Fig. 5: CASP8(C362S) activates the ASC inflammasome in IECs.
From: Caspase-8 is the molecular switch for apoptosis, necroptosis and pyroptosis
a, Count of Paneth cells (per crypt per mouse, n = 3). Dots, individual mice. Data are mean ± s.e.m. One-way ANOVA followed by Sidak’s post-analysis. b, Cytokine array (AYOXXA Lunaris) to detect the indicated cytokines in ileal lysates derived from 5-week-old mice (left) or P1 neonates (right). Dots and circles, individual mice. Data are mean ± s.e.m. One-way ANOVA followed by Turkey’s post-analysis. c, d, Ponceau staining of ileal lysates from 5-week-old mice (n = 2) (c) and P1 neonates (n = 1) (d) (Fig. 3b, c). Lanes, individual mice. e, Western blot analysis of CASP8WT/WT and CASP8−/− HCT-116 and HEK293T CRISPR–Cas9 cell clones. Results are representative of one individual experiment. f, Western blot analysis of the soluble and insoluble fraction of CASP8−/− HCT-116 clone 2 with overexpression of either human wild-type caspase-8 or CASP8(C360S) after treatment with IDN-6556 (20 µM), Nec-1 (10 µM) or both (IDN-6556 and Nec-1) for 14 h. Results are representative of two individual experiments.
