Extended Data Fig. 3: E–PG neuron pairs recorded sequentially from the same brain.

a, Two biocytin filled dendrites (green) from sequentially recorded E–PG neurons that innervate adjacent wedges within the ellipsoid body. Neuropil reference marker is shown in grey (anti-nc82 antibody). Images are maximum intensity z-projections. Scale bar, 10 μm. The schematic shows the approximate position of ellipsoid body and E–PG dendrites from a coronal view of the fly brain. b, c. Heading tuning (red, measured in VR) and visual receptive field (blue, measured with random flashes) from sequentially recorded E–PG pairs from two example flies. Dendritic locations of the recorded neurons are green in the ellipsoid body schematic above each set of plots. In both cases, by chance, the two dendrites were physically adjacent. In both cases, adjacent E–PG neurons from the same fly exhibited similar visual receptive fields and heading tuning curves, supporting the conclusion that adjacent E–PG cells typically receive inhibition from adjacent regions of visual space and represent adjacent heading directions. Comparing the visual receptive field and the heading tuning curve for each neuron yielded correlation coefficients (Pearson’s) of 0.76 (fly 1 neuron 1), 0.90 (fly 1 neuron 2), 0.95 (fly 2 neuron 1) and 0.65 (fly 2 neuron 2). For experiments shown in this figure, we used UAS-mCD8::GFP/UAS-mCD8::GFP; R60D05-Gal4/R60D05-Gal4 flies.