Extended Data Fig. 6: Darobactin disrupts the outer membrane and causes lysis of E. coli.
From: A new antibiotic selectively kills Gram-negative pathogens
E. coli MG1655 cells were placed on top of an agarose pad that contained darobactin and the fluorescent dyes FM4-64—to stain the membrane (false-coloured in magenta)—and Sytox Green—to show membrane permeabilization (false-coloured in green). E. coli MG1655 cells were observed over time at 37 °C under the microscope. For each indicated time point, representative panels show the killing progression of E. coli MG1655 with darobactin. White arrows highlight membrane blebbing; orange arrows highlight swelling and lysis. Scale bars, 5 μm. This figure is representative of three biologically independent experiments performed with similar results.
