Extended Data Fig. 2: NMR assignments of darobactin.

a, ¹H, ¹³C and ¹⁵N NMR chemical shifts (ppm) for darobactin. ^†Owing to overlap with a residual water peak at 4.6 ppm, the multiplicity and J coupling values were from a different ¹H NMR spectrum of darobactin in water:deuterated acetonitrile (2:1, v/v). ^‡Two partially overlapping peaks were observed at 131.79 ppm and 131.83 ppm. b, Structure of darobactin with numbering for NMR assignments. c, Key ROESY correlations (top) and three-dimensional model of darobactin (bottom).