Extended Data Fig. 6: Fascia but not dermal matrix steers into wounds.
a, Matrix tracing in chimeric grafts. b, Grafts at 7 dpw immunolabelled for collagen I, III and VI. Representative image of three biological replicates. c, Label coverage fraction from total collagens in the wound at defined time points. Data are mean ± s.e.m.; n = 4 (7 dpw) and 9 (14 dpw) sections analysed from 3 biological replicates. Unpaired two-tailed t-test, 95% CI. d, Wounds at 14 dpw immunolabelled for collagens. Representative images of three biological replicates. e–f, Higher magnification of the insets in d. g, h, Double matrix tracing in deep-injured dermal EPF-traced grafts at 7 dpw. Representative image of three biological replicates. i, j, Double matrix tracing in deep-injured fascia EPF-traced grafts at 14 dpw. Representative image of three biological replicates. k, l, Double matrix labelling in superficial-injured dermal EPF-traced grafts at 14 dpw immunolabelled for collagens. Representative image of three biological replicates. Dotted lines delimit the wound. Arrowheads mark the original injury. Continuous lines delimit the epidermis dermis margin. Scale bars, 500 μm (b), 100 μm (d–f) and 200 μm (h, j, l).
