Extended Data Fig. 7: Fascia matrix forms the eschar and is remodelled in the wound.
a, Left, in situ matrix tracing and collagen I, III and VI immunolabelling at defined time points after wounding. Representative images of three biological replicates. Right, subsampled fractal dimension maps of the FITC signal of uninjured tissue and at 3 and 7 dpw, and the collagen signal at 14 dpw. b, Matrix label coverage from total collagen I, III and VI signal in the wound. Data are mean ± s.e.m.; n = 3 (uninjured), 4 (3 dpw), 7 (7 dpw) and 4 (14 dpw) sections analysed from 3 biological replicates. One-way ANOVA, Tukey’s multiple comparisons. c, d, Average fractal dimension (c) and lacunarity (d) from subsampled maps. Data are mean ± s.e.m.; n = 5 (uninjured), 5 (3 dpw), 8 (7 dpw) and 3 (14 dpw) images analysed from 3 biological replicates. One-way ANOVA, Tukey’s test, 95% CI. e, Scatter plot of average fractal dimension and lacunarity values. f, In situ matrix tracing and SELP immunolabelling at defined time points after wounding. Representative images of three biological replicates. The broken line separates dermis from fascia. Dotted lines indicate the wound. Scale bars, 200 μm.
