Extended Data Fig. 2: Fascia EPFs traverse PC.
a, Gating strategy for fibroblasts analysis. b, Percentages of fibroblasts (Lin−) and lineage-positive cells in fascia and dermis. Data are mean ± s.e.m.; n = 4 independent experiments. Unpaired two-tailed t-test, 95% CI. c, Scatter plots of EPFs (GFP+Lin−) and ENFs (TdTomato+Lin−) in fascia and dermis. Representative plots of three independent experiments. d, EPF and ENF fractions in fascia and dermis. Data are mean ± s.e.m.; n = 4 independent experiments. Two-way ANOVA, multiple comparison Tukey’s test, 95% CI. e, Endothelial cell (PECAM1+), lymphatic cell (LYVE1+), macrophages (F4/80+) and nerve cell (NGFR+) fractions in fascia and dermis. Data are mean ± s.e.m.; n = 3 biological replicates. Two-way ANOVA, multiple comparison Tukey’s test, 95% CI. f–k, Representative images of 3D-rendered En1cre;R26mTmG or En1cre;R26VT2/Gk3 back skin fascia from at least three biological replicates. f, Lateral view (left) and cross-sections (right) of adult fascia. g, Top view (ventral side up) of neonate back skin. h, Top side view (top) and lateral cross-section (bottom) at the forelimb junction showing EPF traversing the PC. i, Top view at a muscle breach showing EPFs in both locations. j, Top view at a muscle opening where nerves pass through and polyclones of EPFs reside. k, Top view (top, epidermis side up) and lateral cross-section (bottom) of an adult superficial wound (3 dpw). Brocken lines delimit PC. Dotted lines delimit the epidermis. Scale bars, 1,500 μm (g), 100 μm (f, i, j) and 500 μm (k); v, vessels; nb, nerve bundles.
