Extended Data Table 3 Lifespan assays of L2ox and L2red subpopulations following transient exposure to oxidizing or reducing conditions

From: Developmental ROS individualizes organismal stress resistance and lifespan

  1. Worms were sorted into liquid medium, exposed to paraquat or NAC for 10 h and then returned to NGM plates for lifespan assessment. P values for Kaplan–Meier survival analysis were calculated on the basis of the log-rank (Mantel–Cox) method unless noted otherwise. Samples were compared with L2ox subpopulations or L2mixed populations. Maximum lifespan was defined as the lifespan of the last 10% of the population.
  2. *Percentage change based on mean lifespan.
  3. †Percentage change based on maximum lifespan.
  4. ‡P value for survival analysis calculated based on the Gehan–Breslow–Wilcoxon method.
  5. §Grx1–roGFP2 ratio of L2ox and L2red subpopulations at the L2 stage, based on BioSorter analysis during sorting. Data are mean ± s.e.m. n, number of worms. Two-sided unpaired t-test.
  6. ‖Sample compared with the reduced subpopulation.
  7. ¶Lifespan assays performed in the lifespan machine (see Methods).
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