Extended Data Fig. 1: Expression of meristematic-zone marker and transcriptome analysis upon RGF1 treatment.
From: RGF1 controls root meristem size through ROS signalling
a, b, Confocal images of HPY2-GFP roots 24 h after treatment with water (mock; a) or 20 nM RGF1 (b). Seedlings were grown on MS plates for seven days before treatment. Left, PI-stained roots; right, GFP signals. White and blue arrowheads indicate the junction between the meristematic and elongation zones. Scale bar, 50 µm. c, Area of HPY2-GFP expression (in µm2; n = 8 independent roots; P < 2.1 × 10−7). Bar graphs show means. Error bars are ± s.d. Dots indicate each data point. P values are calculated by two-sided Student’s t-test. d, Method of RNA extraction following RGF1 treatment.
