Extended Data Fig. 4: Localization of gPLT2–YFP, NBT and H2O2-BES-Ac staining after RGF1 treatment.
From: RGF1 controls root meristem size through ROS signalling
a–t, Localization of gPLT2–YFP (a–h), NBT staining (i–n) and H2O2-BES-Ac staining (o–t), 4 h after treatment with water (mock; a) or 100 nM RGF1 (b), 6 h after treatment with water (mock; c, i, o) or 100 nM RGF1 (d, j, p), 8 h after treatment with water (mock; e, k, q) or 100 nM RGF1 (f, l, r), or 10 h after treatment with water (mock; g, m, s) or 100 nM RGF1 (h, n, t). Blue arrowheads indicate the junction between the meristematic and elongation zones. Scale bar, 50 µm. Seedlings were grown on MS agar plates for seven days before treatment. Experiments were independently repeated three times with similar results.
