Extended Data Fig. 8: Il17ra expression in the S1DZ of PBS and MIA offspring and further behavioural analyses of S1DZ IL-17a rescue of sociability in MIA offspring and monogenic mutant mice.
From: IL-17a promotes sociability in mouse models of neurodevelopmental disorders
a, Representative images of Il17ra expression in the S1DZ of PBS and MIA offspring. Scale bar, 1 mm. b, Quantification of Il17ra expression within the S1DZ of MIA offspring according to cortical layer. n = 6, from 2 independent experiments. c, Quantification of overall Il17ra expression in the S1DZ of PBS and MIA offspring. PBS offspring, n = 8; MIA offspring, n = 6; from 2 independent experiments. d–h, Further behavioural analyses of experiments described in Fig. 3f. Time spent investigating social (S) versus inanimate (I) objects (e), total interaction time (f), time spent in social (S), centre (C) or inanimate (I) chamber (g), and distance travelled (h). PBS offspring + vehicle, n = 11; PBS offspring + IL-17a, n = 12; MIA offspring + vehicle, n = 14; MIA offspring + IL-17a, n = 10; wild type + vehicle, n = 11; wild type + IL-17a, n = 11; Cntnap2 mutant + vehicle, n = 8; Cntnap2 mutant + IL-17a, n = 10; Fmr1 mutant + vehicle, n = 9; Fmr1 mutant + IL-17a, n = 11; from 6 independent experiments. All n values refer to the number of mice used. Statistics calculated by unpaired two-tailed t-test (c), two-way ANOVA with Sidak’s (e) or Dunnett’s (g) post-hoc tests, or two-way repeated-measures ANOVA with Sidak’s post-hoc test (f, h). Graphs are mean ± s.e.m.
