Extended Data Fig. 7: Acute inhibitor or knockdown treatment of tail-bud and dissociated PSM-cell cultures.
From: Coupling delay controls synchronized oscillation in the segmentation clock
a–c, Expression of the Hes7-Achilles reporter in wild-type tail-bud tissue cultures treated with DMSO control (grey bars) or the Notch inhibitor DAPT (red bars). Period (a), amplitude (b) and synchrony (c) of HES7 oscillations were quantified. Error bars indicate s.e.m. *P < 0.05, unpaired t-test. The number of cells examined (n) is indicated. ****P < 0.0001, Rayleigh test. d, Kuramoto order parameter calculated using Achilles–HES7 oscillation phase quantified in c (time (t) = 400–800 min). Error bars indicate s.e.m. *P < 0.05, unpaired t-test. e, f, Expression of Hes7-Achilles reporter wild-type tail-bud tissue cultures treated with scrambled shRNA (shScramble) (grey bars) or two different shRNAs against Lfng (shLfng-1 and shLfng-2) (blue bars). Synchrony (e) and Kuramoto order parameter (f, t = 600–900 min) of HES7 oscillations were quantified. The number of cells examined (n) is indicated. ****P < 0.0001, Rayleigh test (e). Error bars indicate s.e.m. *P < 0.05, unpaired t-test (f). g, h, Expression of Hes7-Achilles reporter in dissociated PSM cell cultures treated with DAPT. Period (g) and amplitude (h) of HES7 oscillations were quantified. Error bars indicate s.e.m.
