Extended Data Fig. 2: Purification, cryo-EM data imaging and processing of the TT-OAD2–GLP-1R–Gs complex.
From: Activation of the GLP-1 receptor by a non-peptidic agonist
a, Representative elution profile of Flag-purified complex on Superdex 200 Increase 10/30 SEC. b, Representative micrograph of the TT-OAD2–GLP-1R–Gs complex. Red circles highlight examples of individual particles. c, Two-dimensional class averages of the complex in maltose-neopentyl glycol (MNG) micelle. d, Cryo-EM data processing workflow. e, Gold-standard Fourier shell correlation (FSC) curves, showing the overall nominal resolution at 3.0 Å. f, 3D histogram representation of the Euler angle distribution of all the particles used for the in the reconstruction overlaid on the density map drawn on the same coordinate axis (map is coloured according to local resolution as in g). g, Cryo-EM density map coloured according to resolution. Left, map with the GLP-1R ECD masked; right, map including the ECD of GLP-1R.
